CHD1L Knockout cell line (A549)

CHD1L Knockout cell line (A549) Order

Catalog#

YKO-HS13488

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

CHD1L Knockout cell line (A549)

Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:4

Species

Human

Gene Name

CHD1L

Gene ID

9557

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90% F12K+10% FBS

Target region

Knockout region: Exon 1 (based on transcript CHD1L-202)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CHD1L gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the A549 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CHD1L gene knockout CHD1L Knockout cell line (A549) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

CHD1L Knockout cell line (A549)

Catalog#

YKO-HS13488

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Related Popular Products

Wild-type Cell Lines

Human Non-small Cell Lung Cancer Cell Line(A549)
inquiry More details

Catalog#: YC-C016

Size: 1*10^6

Instruction:

Gene Knockout kit

EZ-editor™Gene Knockout kit
inquiry More details

Catalog#: MY-001

Size: -

Instruction:

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium
inquiry More details

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

KO Cell Lines

CHD1L Knockout cell line (HCT 116)
inquiry More details

Organism: Human

Gene Name: CHD1L

Gene ID: 9557

Size: 1*10^6

Catalog#: YKO-H598

YKO-H598(HCT 116)
CHD1L Knockout cell line (HeLa)
inquiry More details

Organism: Human

Gene Name: CHD1L

Gene ID: 9557

Size: 1*10^6

Catalog#: YKO-HS13491

YKO-HS13491(HeLa)
CHD1L Knockout cell line (HEK293)
inquiry More details

Organism: Human

Gene Name: CHD1L

Gene ID: 9557

Size: 1*10^6

Catalog#: YKO-HS13489

YKO-HS13489(HEK293)

Related Popular Products

Wild-type Cell Lines

Human Non-small Cell Lung Cancer Cell Line(A549)

Price (USD): -

Catalog#: YC-C016

Size: 1*10^6

Instruction:

inquiry More details

Gene Knockout kit

EZ-editor™Gene Knockout kit

Price (USD): 998

Catalog#: MY-001

Size: -

Instruction:

inquiry More details

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

Price (USD): -;-

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

inquiry More details

KO Cell Lines

CHD1L Knockout cell line (HCT 116)

Organism: Human

Gene Name: CHD1L

Gene ID: 9557

Size: 1*10^6

Catalog#: YKO-H598

inquiry More details
CHD1L Knockout cell line (HeLa)

Organism: Human

Gene Name: CHD1L

Gene ID: 9557

Size: 1*10^6

Catalog#: YKO-HS13491

inquiry More details
CHD1L Knockout cell line (HEK293)

Organism: Human

Gene Name: CHD1L

Gene ID: 9557

Size: 1*10^6

Catalog#: YKO-HS13489

inquiry More details

CHD1L Knockout cell line (A549)

Ubigene